Biosensors Dynamic HK-GFP-1 Akwụkwọ ntuziaka onye ọrụ GFP Capture

HK-GFP-1 GFP ngwa nkpu

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Nkọwapụta:

• Aha ngwaahịa: heliX + GFP CAPTURE KIT nwere ihe na-acha uhie uhie Ra
• Onye nrụpụta: Dynamic Biosensors GmbH
• Ụdị: HK-GFP-1 v3.1
• Akụkụ ndị bụ isi:
  • 2 tụrụ nwere usoro arịlịka abụọ dị iche iche maka DNA-ederede
    na-agwa okwu
  • Ntụpọ 1 arụrụ ọrụ site na molikula weghara
  • Spot 2 ejiri dị ka ntụaka oge

Nkọwa ngwaahịa:

Nọmba iwu: HK-GFP-1

Ọdịnaya na Ozi Nchekwa:

Ihe onwunwe	Ntinye uche
GFP protein njikọ - Ligand strand Green	500 nM
eriri nkwụnye 1 - Ra Black	400 nM
eriri nkwụnye 2 – Ra – lfs White	200/250 nM

Ntuziaka ojiji:

Usoro nha:

1. GFP -tagged ligand na-ejide n'elu n'oge
   nha ọsọ.
2. Tụọ ihe nyocha na-ejikọta kinetics ma ọ bụ mgbanwe mgbanwe
   n'elu njide nyocha.
3. Megharịa elu elu site n'itinye ngwọta pH dị elu na
   weghachi ya na steeti izizi.

Nkwadebe:

Ntọala nyocha na heliOS:

1. Gaa na heliOS> mepụta New Assay Workflow> tinye omenala
Nyocha > Ibu Kinetics nwere Capture> gbanwee paramita dabere
na mkpa gị ma mee nyocha ahụ.

Akụkụ nyocha atụnyere:

Ọnụego ọsọ, oge, ike LED, wdg, dị n'ime nyocha heliOS.
Maka nkọwa ndị ọzọ, kpọtụrụ support@dynamic-biosensors.com.

Ajụjụ:

Ọnụọgụ ịtụ bara uru:

• Chip nwere ntụpọ nchọpụta 2: Nyeghị iwu ADP-48-2-0
• Passivation nke mgbawa elu: Nye iwu Ọ dịghị SOL-PAS-1-5
• Mweghachi nke mgbawa elu: Nye iwu enweghị SOL-REG-1-5

Ozi mkpọtụrụ:

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich Germany

Ụgwọ nke ụlọ ọrụ Bruker Scientific LLC
40 Manning Road, Mamming Park Billerica, MA 01821 USA

Ozi ịtụ:
order@dynamic-biosensors.com
Nkwado ndị teknuzu:
support@dynamic-biosensors.com

Mara: Maka nkọwa teknụzụ zuru ezu na
ozi ndị ọzọ, rụtụ aka na akwụkwọ ntuziaka ngwaahịa na
www.dynamic-biosensors.com.

"'

heliX +
Akwụkwọ ntuziaka onye ọrụ
GFP CAPTURE KIT
na-acha uhie uhie Ra
Biosensors Dynamic GmbH HK-GFP-1 v3.1

Atụmatụ igodo
Emebere ngwa a maka ijide GFP-tagged ligands na-eji protein ejikọta GFP. Dakọtara na mgbakwụnye heliX®. · Gụnyere eriri nkwụnye na eriri Ligand emebere ya na protein njikọ GFP maka mmụgharị 20. N'ihi na functionalization nke Spot 1 na Spot 2. · nkwụnye eriri 1 na 2 na-ebu a moderately hydrophilic red dye (Ra) na otu nti net ụgwọ.
HelX® Adapter Chip Overview
2 tụrụ nwere usoro arịlịka abụọ dị iche iche maka adreesị nwere DNA. A na-arụ ọrụ ntụpọ 2 site na molikula weghara ebe a na-eji Spot 1 dị ka ntụaka oge.

Nkọwa ngwaahịa

Nọmba iwu: HK-GFP-1

Isiokwu 1. Ọdịnaya na Ozi Nchekwa

Ihe onwunwe

Okpu

GFP na-ejikọta protein [1] - Ligand strand Green

eriri nkwụnye 1 - Ra

Nwa

eriri nkwụnye 2 – Ra – lfs

Ọcha

Ntinye uche 500 nM 400 nM 200/250 nM

Ọnụ ego 2 x 100 µL 2 x 100 µL 2 x 200 µL

Maka iji naanị nyocha.
Ngwaahịa a nwere ndụ nchekwa nwere oke, biko hụ ụbọchị ngwụcha na label. Mgbe nkwadebe nke njikere iji ngwọta ụbọchị njedebe bụ ọnwa 3.

Ihe nchekwa TE40 [2] TE40 [2] TE40 [2]

Nchekwa 2-8°C -20°C -20°C

HK-GFP-1 v3.1

www.dynamic-biosensors.com

Ibe 1 nke 4

Usoro ọrụ nke heliX® GFP – nyocha njide

1. Arịlịka arịlịka (ssDNA) nke a na-agaghị emegharị n'elu HelX® Adapter Chip ka ejiri eriri DNA agbakwunyere gbanwetụrụ na protein GFP.
2. GFP-tagA na-ejide ged ligand nke mmasị n'elu n'oge ọsọ nha. 3. Ntụle nke analyte binding kinetics ma ọ bụ mgbanwe mgbanwe n'elu njide nyocha. 4. Mweghachi nke elu site na ntụtụ nke ngwọta pH dị elu. Igwe mgbawa na-alaghachi na steeti izizi. Nzọụkwụ a nwere ike ịbụ
sochiri hyridization ọhụrụ nke ligand ọhụrụ nwere protein na-ejikọta GFP.
Nkwadebe
1. Gwakọta protein 100 µL GFP - eriri Ligand nwere eriri nkwụnye 100 µL 1 - Ra. 2. Tinye ihe ngwọta nke nzọụkwụ 1 na RT na 600 rpm maka 30 min iji hụ na ngwakọ zuru ezu. 3. Gwakọta eriri nkwụnye 200 µL 2 - Ra - lfs na s.ample mgbe nzọụkwụ 2 gasịrị.
Ihe ngwọta (400 µL na mkpokọta) dị njikere iji maka ịrụ ọrụ biochip.
Biko wụsa ma chekwaa njikere iji ngwọta na 2-8 Celsius. Jiri ya n'ime ọnwa 3.
Ngwa ahụ nwere ihe maka nkwadebe nke abụọ dị njikere iji ngwọta na 400 µL nke ọ bụla.
Ntọlite ​​​​assay na heliOS
Gaa na heliOS> mepụta New Assay Workflow> tinye Custom Assay> Ibu Kinetics with Capture> gbanwee paramita dabere na mkpa gị wee mee nyocha ahụ.
Atụmatụ nyocha akwadoro (dịka ọmụmaatụ, ọnụego ọsọ, oge, ike ikanam, wdg) dị n'ime nyocha heliOS.
Maka ajụjụ ndị ọzọ, biko kpọtụrụ ndị otu nkwado na support@dynamic-biosensors.com

HK-GFP-1 v3.1

www.dynamic-biosensors.com

Ibe 2 nke 4

Ọnụọgụ ọnụ ahịa bara uru
Tebụl 2. Nye iwu nọmba aha ngwaahịa heliX® Adapter Chip 10x Passivation solution Regeneration solution

Ikwu Chip nwere ntụpọ nchọpụta 2 Maka passivation nke mgbawa elu Maka imeghari elu mgbawa

Nye iwu mba ADP-48-2-0 SOL-PAS-1-5 SOL-REG-1-5

HK-GFP-1 v3.1

www.dynamic-biosensors.com

Ibe 3 nke 4

Kpọtụrụ

Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany

Bruker Scientific LLC 40 Manning Road, Mamming Park Billerica, MA 01821 USA

Nye ozi order@dynamic-biosensors.com Nkwado nka na ụzụ support@dynamic-biosensors.com
www.dynamic-biosensors.com
A na-arụ ọrụ na ibe na-arụ ọrụ na Germany. ©2025 Dynamic Biosensors GmbH maka iji nyocha naanị. Ọ bụghị maka ojiji na usoro nyocha ụlọ ọgwụ.

[1] GFP protein jikọrọ ọnụ bụ ngwaahịa ChromoTek GmbH [2] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 M EDTA, 50 M EGTA

HK-GFP-1 v3.1

www.dynamic-biosensors.com

Ibe 4 nke 4

Akwụkwọ / akụrụngwa

Ngwa ihe eji eme ihe biosensors HK-GFP-1 GFP [pdf] Akwụkwọ ntuziaka onye ọrụ HK-GFP-1 GFP Nkịtị Njikere, HK-GFP-1, GFP Capture Kit, Ngwa Njide

Ntụaka

• Akwụkwọ ntuziaka onye ọrụ