dynamic BIOSENSORS heliXcyto Normalisation Solution

Atụmatụ igodo

• Maka nhazi nke akara fluorescent na Spot 1 na Spot 2 nke mgbawa heliX cyto.
• Na-enyere aka ntutụ aka ziri ezi nke mgbaàmà fluorescent na-acha uhie uhie ma ọ bụ akwụkwọ ndụ akwụkwọ ndụ n'oge nha RT-IC
• Dakọtara na ibe cyto heliX niile
• Ngwọta Normalisation-Ra na-ebu ihe na-acha uhie uhie hydrophilic nke nwere otu ụgwọ net dị mma
• Ngwọta Normalisation-Ga na-ebu acha anụnụ anụnụ hydrophilic nwere otu ụgwọ net na-adịghị mma

Nkọwa ngwaahịa

Nọmba ịtụ ahịa: Ọ BỤGHỊ-0

Tebụl 1. Ọdịnaya na Ozi Nchekwa

Ihe onwunwe	Okpu	Ntinye uche	Ọnụ ego	Nchekwa
Ngwọta Normalisation-Ra	oroma	10 µM	3 x 100 µL	-20 Celsius
Ngwọta Normalisation-Ga	Akwụkwọ ndụ akwụkwọ ndụ	10 µM	3 x 100 µL	-20 Celsius

Maka iji naanị nyocha.

Ngwaahịa a nwere ndụ nchekwa nwere oke, biko hụ ụbọchị ngwụcha na label.

Nkwadebe

Họrọ agba ngwọta nkịtị (Ga ma ọ bụ Ra) dabara na agba akara nyocha gị. Gwakọta 10 µM ngwọta ngwaahịa nkịtị ka ọ bụrụ itinye uche na-arụ ọrụ yana ihe nchekwa na-agba ọsọ.

Enwere ike ịchọta olu ikpeazụ achọrọ maka nnwale ahụ na heliOS sampna tray view nke nyocha.

Nleba anya nke ngwọta normalization kwesịrị ịdị nhata kwekọrọ na ntinye fluorophore na mkpokọta nyocha kachasị elu ka a tụrụ. Enwere ike gbakọọ nke a site na iji nha nha:

: Ntinye uche nke ngwọta normalization na agba a chọrọ
: Ntụkwasị nke agba na ngwọta nyocha akpọrọ
: Ntụle nyocha kachasị elu nke kwesịrị ịtụ
: ogo nke labeling (oke nke agba ka nyocha)

Enwere ike ịchekwa ihe ngwọta a gwakọtara na 2-8 Celsius C ruo ụbọchị 7.

Mkpa: Ejikọtala Normalisation solution-Ra na Normalisation solution-Ga ọnụ, ọ gwụla ma ị na-ahazi nha Dual Agba (ngụkọta nke akwụkwọ ndụ akwụkwọ ndụ na ọwa uhie).

Ngwa ndetu

Na nha RT-IC, akara fluorescent nke ngwọta nkịtị kwesịrị ịdị na a yiri nso dị ka mgbaàmà kachasị elu na-abịa site na bound analyte (raw data).

Mgbama fluorescent zuru oke na-adabere na nhazi nhazi nhazi nhazi yana ike mkpali etinyere na nha. A ga-ahọrọ ike mkpali ahụ dabere na oke ndị a:

a. Ntinye uche nke fluorophore na ngwọta nyocha:
Ntinye uche nke fluorophore dabere na nleba anya nyocha ejiri na nha yana ogo akara nke nyocha. Maka nnukwu DOL na mkpokọta nyocha dị elu, enwere ike ịchọ iweda ike mkpali.

b. Mgbama njide tụrụ anya:
Ebumnobi egosipụtara nke ukwuu na cell nwere ike jikọta mkpụrụ ndụ ihe nketa akara nyocha. Ọ bụrụ na ebumnobi egosipụta oke oke, enwere ike ịtụ anya mgbama njikọ siri ike. Iji zere mmechi mkpuchi, enwere ike ịtụle iweda ike mkpali.

c. Ụdị mgbawa:
Ụdị mgbawa dị iche iche nwere ndabere fluorescent dị iche iche. Ka ọnyà ndị ahụ buru ibu na ọnyà ndị ọzọ na mgbawa, ka mgbaàmà ndabere dị elu. Ya mere, ibe L5 nwere ike ịchọ ike mkpali dị ala karịa etinyere na ibe M5.

Maka mmalite nke ike mkpali na ụkpụrụ. Nleba anya ngwọta ga-eji na nnwale RT-IC, biko rụtụ aka na Tebụl 2.

Tebụl 2. Mmekọrịta nke mkpokọta fluorophore, nhazi nhazi nhazi nhazi, na ike mkpali dabara maka mgbawa heliX cyto M5.

Analyte dye conc. = nyocha conc x DOL	Ike mkpali	Ngwọta nhazi nhazi nke uche	Dilution Normalisation ngwọta
25 nM	0.5	25 nM	1:400
50 nM	0.3	50 nM	1:200
100 nM	0.2	100 nM	1:100
300 nM	0.1	300 nM	1:33
500 nM	0.08	500 nM	1:20
1 µM	0.05	1 µM	1:10
2.5 µM	0.02	2.5 µM	1:4

Mara: Tebụl a bụ maka ntụzịaka gị. Otú ọ dị, mgbaàmà ikpeazụ e dekọrọ na heliX cyto na-adabere n'ọtụtụ ihe. Ya mere, a ga-achọ ụfọdụ njikarịcha maka sistemụ ọ bụla.

Kpọtụrụ

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81379 Munich	Woburn, MA 01801
Germany	USA

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dynamic BIOSENSORS heliXcyto Normalisation Solution [pdf] Akwụkwọ ntuziaka onye ọrụ NOR-0, HelXcyto Normalisation Solution, heliXcyto, Ngwọta Normalisation, Ngwọta

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