dynamic BIOSENSORS heliX gbakwunyere Adapter Strand 2

Atụmatụ igodo

• eriri nkwụnye 2 maka ịrụ ọrụ nke heliX® Adapter Chip Spot 2.
• Dakọtara na HelX® Adapter Chip.
• Gụnyere eriri nkwụnye maka nrụpụta 50.
• Kwesịrị ekwesị maka MIX&RUN sample nkwadebe.
• Ụdọ nkwụnye 2 na-ebu ihe na-acha uhie uhie hydrophobic (Rc) na ụgwọ net na-anọpụ iche.

HelX® Adapter Chip Overview

2 tụrụ nwere usoro arịlịka abụọ dị iche iche maka adreesị nwere DNA.

Nkọwa ngwaahịa

Nọmba iwu: AS-2-Rc-lfs
Isiokwu 1. Ọdịnaya na Ozi Nchekwa

Ihe onwunwe	Okpu	Ntinye uche	Ọnụ ego	Ihe nchekwa	Nchekwa
eriri nkwụnye 2 - Rc - lfs	Ọcha	200/250 nM	5 x 200 µL	TE40 [1]	-20°C

Maka iji naanị nyocha.
Ngwaahịa a nwere ndụ nchekwa nwere oke, biko hụ ụbọchị ngwụcha na label.
Iji zere ọtụtụ okirikiri ifriizi, biko tinye nanolever.

Nkwadebe | Mix&RUN

Ngwọta nke ngwakọ nke nkwụnye na eriri ligand:

1. Mix Adapter strand 1 – Rc (400 nM) na conjugated Ligand strand (500 nM) na 1: 1 ratio (v/v).
2. Tinye ihe ngwọta nke nzọụkwụ 1 na RT na 600 rpm maka 30 min iji hụ na ngwakọ zuru ezu.
3. Ngwakọta ngwọta nke nzọụkwụ 2 na nkwụnye nkwụnye 2 - Rc - lfs (200 nM) na 1: 1 ratio (v / v).
   Ngwọta adịla njikere iji maka ịrụ ọrụ biochip.
   Nkwụsi ike nke ngwọta metụtara nkwụsi ike nke molecule ligand.

Tebụl 2. Ihe mgbakwunye maka ịrụ ọrụ nke ntụpọ 1 na ntụpọ ntụaka 2.

Ihe onwunwe	Ntinye uche	Ihe nchekwa	Aha ngwaahịa emetụtara	Iwu Mba
eriri nkwụnye 1 - Rc	400 nM	TE40 [1]	eriri nkwụnye 1 na-acha uhie uhie Rc	AS-1-Rc
Ligand eriri na-ebu eriri conjugated	500 nM	2P] E40 [	Ngwakọta njikọ heliX® Amine 1	HK-NHS-1

Example
Olu achọrọ maka ịrụ ọrụ 3: 100 μL na ntinye ikpeazụ nke 100 nM.

Vial 1		Vial 2
eriri nkwụnye 1 - Rc (400 nM)	Ejikọtara Njikọ Njikọ (500 nM)	eriri nkwụnye 1 - Rc - lfs (200/250 nM)
25 µL	25 µL	50 µL

Mgbe oge ntinye, gwakọta vial 1 na vial 2 iji nweta 100 μL nke ngwọta DNA dị njikere iji.

Kpọtụrụ

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany

Ọnọdụ nke Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA

Ozi ịtụ order@dynamic-biosensors.com
Nkwado ndị teknuzu support@dynamic-biosensors.com
www.dynamic-biosensors.com

A na-arụ ọrụ na ibe na-arụ ọrụ na Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Ikike niile echekwabara.

1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Ọ bụrụ na protein anaghị akwụsi ike na PE40 (TE40, HE40), biko lelee ndakọrịta ihe nchekwa na mpempe ndakọrịta switchSENSE®.

www.dynamic-biosensors.com

Akwụkwọ / akụrụngwa

	dynamic BIOSENSORS heliX gbakwunyere Adapter Strand 2 [pdf] Akwụkwọ ntuziaka onye ọrụ AS-2-Rc-lfs v5.1, heliX gbakwunyere ihe nkwụnye Strand 2, nkwụnye eriri 2, Strand 2
	dynamic BIOSENSORS heliX gbakwunyere Adapter Strand 2 [pdf] Akwụkwọ ntuziaka onye ọrụ heliX gbakwunyere ihe nkwụnye ihe nkwụnye 2, heliX gbakwunyere, nkwụnye eriri 2, Strand 2

Ntụaka

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