dynamic BIOSENSORS heliX gbakwunyere Adapter Strand 1

Nkọwapụta:

• Aha ngwaahịa: heliX+
• Ụdị: ADAPTER STRAND 1 nwere ihe na-acha uhie uhie Ra
• Onye nrụpụta: Dynamic Biosensors GmbH & Inc.
• Nọmba iwu: AS-1-Ra
• Ntinye uche: 400 nM
• Nchekwa: Okpu ojii, Buffer TE40
• Maka iji naanị nyocha

Nkọwa ngwaahịa:

Ejiri heliX + ADAPTER STRAND 1 mee ya na-acha uhie uhie Ra ma nwee ntụpọ 2 nwere usoro arịlịka 2 dị iche iche maka ntinye ederede DNA. Ọ na-abịa na ntinye nke 400 nM ma ezubere ya maka ebumnuche nyocha.

Ntuziaka ojiji:
Nkwadebe | Mix&gbaa:

1. Mix Adapter strand 1 – Ra (400 nM) na conjugated Ligand strand (500 nM) na nha 1: 1 (v/v).
2. Tinye ihe ngwọta site na nzọụkwụ 1 na ụlọ okpomọkụ na 600 rpm maka nkeji 30 iji hụ na ngwakọ zuru ezu.
3. Gwakọta ihe ngwọta site na nzọụkwụ 2 na eriri nkwụnye 2 - Ra - lfs (200 nM) na nha 1: 1 (v / v).
4. Ihe ngwọta adịla njikere ugbu a iji mee ihe maka ịrụ ọrụ biochip.

Rịba ama: Nkwụsi ike nke ngwọta na-adabere na nkwụsi ike nke molecule ligand.

Ajụjụ:

1. Ajụjụ: Gịnị bụ ndụ shelf nke heliX+ ADAPTER STRAND 1?
   A: Ngwaahịa a nwere oke nchekwa, biko rụtụ aka na ụbọchị ngwụcha na label.
2. Ajụjụ: Enwere ike ịmegharị heliX+ ADAPTER STRAND 1?
   A: Iji zere ọtụtụ okirikiri ifriizi-thaw, a na-atụ aro ka ị wepụta nanolever.

heliX+
Akwụkwọ ntuziaka onye ọrụ

Ụdọ ada ada 1
na-acha uhie uhie Rb
Dynamic Biosensors GmbH & Inc.
AS-1-Rb v5.1

Atụmatụ igodo

•  eriri nkwụnye 1 – Rb maka ịrụ ọrụ nke heliX® Adapter Chip Spot 1.
• Dakọtara na HelX® Adapter Chip.
• Gụnyere eriri nkwụnye maka nrụpụta 50.
• Kwesịrị ekwesị maka MIX&RUN sample nkwadebe.
• Ụdọ nkwụnye 1 na-ebu ihe na-acha uhie uhie hydrophilic (Rb) na-akwụghị ụgwọ na ụgwọ net na-anọpụ iche.

HelX® Adapter Chip Overview
2 tụrụ nwere usoro arịlịka abụọ dị iche iche maka adreesị nwere DNA.

Nkọwa ngwaahịa

Nọmba iwu: AS-1-Rb
Isiokwu 1. Ọdịnaya na Ozi Nchekwa

Ihe onwunwe	Okpu	Ntinye uche	Ọnụ ego	Ihe nchekwa	Nchekwa
eriri nkwụnye 1 - Rb	Nwa	400 nM	5 x 100 µL	TE401]	-20°C

Maka iji naanị nyocha.

• Ngwaahịa a nwere ndụ nchekwa nwere oke, biko hụ ụbọchị ngwụcha na label.
• Iji zere ọtụtụ okirikiri ifriizi, biko tinye nanolever

Nkwadebe | Mix&RUN

Ngwọta nke ngwakọ nke nkwụnye na eriri ligand:

1.  Mix Adapter strand 1 – Rb (400 nM) na conjugated Ligand strand (500 nM) na 1: 1 ratio (v/v).
2.  Tinye ihe ngwọta nke nzọụkwụ 1 na RT na 600 rpm maka 30 min iji hụ na ngwakọ zuru ezu.
3.  Ngwakọta ngwọta nke nzọụkwụ 2 na nkwụnye nkwụnye 1 - Rb - lfs (200 nM) na 1: 1 ratio (v / v). Ngwọta adịla njikere iji maka ịrụ ọrụ biochip. Nkwụsi ike nke ngwọta metụtara nkwụsi ike nke molecule ligand

Tebụl 2. Ihe mgbakwunye maka ịrụ ọrụ nke ntụpọ 1 na ntụpọ ntụaka 2.

Ihe onwunwe	Ntinye uche	Ihe nchekwa	Aha ngwaahịa emetụtara	Iwu Mba
Ligand eriri na-ebu eriri conjugated	500 nM	P2E40 [	nnwetaX® Amine Njikọ Ngwakọta 1	HK-NHS-1
eriri nkwụnye 1 - Rb - lfs	200/250 nM	TE401]	eriri nkwụnye 2 na-acha uhie uhie Rb prehybridized na eriri na-enweghị ligand	AS-2-Rb-lfs

Example
Olu achọrọ maka ịrụ ọrụ 3: 100 μL na ntinye ikpeazụ nke 100 nM.

Vial 1		Vial 2
eriri nkwụnye 1 - Rb (400 nM)	Ejikọtara Njikọ Njikọ (500 nM)	eriri nkwụnye 1 - Rb - lfs (200/250 nM)
25 µL	25 µL	50 µL

Mgbe oge ntinye, gwakọta vial 1 na vial 2 iji nweta 100 µL nke ngwọta DNA dị njikere iji.

Kpọtụrụ
Dynamic Biosensors GmbH

• Perchtinger Str. 8/10
• 81379 Munich
• Germany

Ọnọdụ nke Dynamic Biosensors, Inc.

• 300 Trade Center, Suite 1400
• Woburn, MA 01801
• USA

Ozi ịtụ order@dynamic-biosensors.com
Nkwado ndị teknuzu support@dynamic-biosensors.com

www.dynamic-biosensors.com
A na-arụ ọrụ na ibe na-arụ ọrụ na Germany. ©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Ikike niile echekwabara.

1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Ọ bụrụ na protein anaghị akwụsi ike na PE40 (TE40, HE40), biko lelee ndakọrịta ihe nchekwa na mpempe ndakọrịta switchSENSE®.

www.dynamic-biosensors.com

Akwụkwọ / akụrụngwa

dynamic BIOSENSORS heliX gbakwunyere Adapter Strand 1 [pdf] Akwụkwọ ntuziaka onye ọrụ AS-1-Ra, AS-2-Ra-lfs, heliX gbakwunyere ihe nkwụnye Strand 1, heliX gbakwunyere, nkwụnye eriri 1, Strand 1

Ntụaka

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