Ihe ngwungwu Strand Biosensors dị ike
Atụmatụ igodo
- Ihe nkwụnye nkwụnye 1 - Ga na nkwụnye eriri 2 - Ga - lfs ( ligand free strand ) maka ịrụ ọrụ nke heliX ® Adapter Chip na Spot 1 na Spot 2, n'otu n'otu.
- Dakọtara na heliX ® Adapter Chip.
- Gụnyere eriri nkwụnye maka nrụpụta 50.
- Kwesịrị ekwesị maka MIX&RUN sample nkwadebe.
- Ụdọ ihe nkwụnye 1 na 2 na-ebu ihe na-acha akwụkwọ ndụ akwụkwọ ndụ hydrophilic (Ga) nke nwere otu ụgwọ net na-adịghị mma.
Ihe nkwụnye ihe nkwụnyeview
2 tụrụ nwere usoro arịlịka abụọ dị iche iche maka adreesị nwere DNA.
Nkọwa ngwaahịa
Nọmba ịtụ ahịa: ASP-1-Ga
Tebụl 1. Ọdịnaya na Ozi Nchekwa
| Ihe onwunwe | Okpu | Ntinye uche | Ọnụ ego | Ihe nchekwa | Nchekwa |
| eriri nkwụnye 1 - Ga | Nwa | 400 nM | 5 x 100 µL | TE40 [1] | -20°C |
| eriri nkwụnye 2 – Ga – lfs | Ọcha | 200/250 nM | 5 x 200 µL | TE40 [1] | -20°C |
Maka iji naanị nyocha.
ngwaahịa ya nwere ndụ nchekwa nwere oke, biko hụ ụbọchị ngwụcha na label.
Iji zere ọtụtụ okirikiri ifriizi, biko tinye lever nano.
Nkwadebe | Gwakọta&gbaa ọsọ
Ngwọta nke ngwakọ nke nkwụnye na eriri ligand:
- Mix Adapter strand 1 - Ga (400 nM) na conjugated Ligand strand (500 nM) na 1: 1 ratio (v / v).
- Tinye ihe ngwọta nke nzọụkwụ 1 na RT na 600 rpm maka 30 min iji hụ na ngwakọ zuru ezu.
- Ngwakọta ngwọta nke nzọụkwụ 2 na nkwụnye nkwụnye 2 - Ga - lfs (200 nM) na 1: 1 ratio (v / v).
Ngwọta adịla njikere iji maka ịrụ ọrụ biochip.
Nkwụsi ike nke ngwọta metụtara nkwụsi ike nke molecule ligand.
Tebụl 2. Ihe mgbakwunye maka ịrụ ọrụ ntụpọ 1 na ntụpọ ntụaka 2.
| Ihe onwunwe | Ntinye uche | Ihe nchekwa | Aha ngwaahịa emetụtara | Iwu Mba |
| eriri Ligand na-ebu ligand jikọtara ọnụ | 500 nM | PE40 [2] | heliX® Amine Njikọ Ngwakọta 1 | HK-NHS-1 |
Example
Olu achọrọ maka ịrụ ọrụ 3: 100 µL na ntinye ikpeazụ nke 100 nM.
| Vial 1 | Vial 2 | |
| eriri nkwụnye 1 – Ga (400 nM) | eriri Ligand jikọtara ọnụ (500 nM) | eriri nkwụnye 2 - Ga - lfs (200/250 nM) |
| 25 µL | 25 µL | 50 µL |
Mgbe oge ntinye, gwakọta vial 1 na vial 2 iji nweta 100 µL nke ngwọta DNA dị njikere iji.
Nkwado ndị ahịa
Kpọtụrụ
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Ụgwọ nke ụlọ ọrụ Bruker Scientific LLC
40 Manning Road, Manning Park
Billerica, MA 01821
USA
Ozi ịtụ order@dynamic-biosensors.com
Nkwado ndị teknuzu support@dynamic-biosensors.com
www.dynamic-biosensors.com
A na-arụ ọrụ na ibe na-arụ ọrụ na Germany.
©2025 Dynamic Biosensors GmbH
Maka Jiri naanị nyocha. Ọ bụghị maka ojiji na usoro nyocha ụlọ ọgwụ.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
[2] Ọ bụrụ na protein anaghị eguzosi ike na PE40 (TE40, HE40), biko lelee ndakọrịta nchekwa na switchSENSE®
akwụkwọ ndakọrịta.
Akwụkwọ / akụrụngwa
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Ihe ngwungwu Strand Biosensors dị ike [pdf] Akwụkwọ ntuziaka onye ọrụ Ihe ngwungwu Strand ihe nkwụnye, ngwugwu Strand, ngwugwu |