Ngwa heliX gbakwunyere Amine Coupling Kit 1, nke a makwaara dị ka HK-NHS-1, bụ ihe ngwọta zuru oke maka njikọta ụmụ irighiri ihe na amines bụ isi maka ebumnuche ịdị ọcha. Ngwa ahụ gụnyere ihe niile dị mkpa yana ntuziaka zuru ezu maka usoro njikọ nzọụkwụ 3 . Edepụtara ihe ndị ọzọ achọrọ maka ojiji, yana mkpachapụ anya dị mkpa iji hụ na nsonaazụ kacha mma.
Chọpụta akwụkwọ ntuziaka onye ọrụ HelX+ Regeneration Solution maka mgbawa elu elu nke Dynamic Biosensors. Mụta maka nkọwapụta ngwaahịa, ntuziaka ojiji, yana ajụjụ maka SOL-REG-1-5. Chekwaa n'ebe dị jụụ, kpọrọ nkụ na-anọghị na ìhè anyanwụ kpọmkwem maka nsonaazụ kacha mma.
Chọpụta akwụkwọ ntuziaka onye ọrụ HK-SA-1 v6.1 Biotin Capture Kit nwere nkọwapụta yana njiri mara maka nhazi nyocha nke ọma. Mụta maka ndụ shelf ngwaahịa, usoro nkwadebe, yana usoro mmeghari mgbawa. Chọgharịa ntuziaka zuru ezu nke Dynamic Biosensors nyere maka iji kacha mma.
Chọpụta akwụkwọ ntuziaka onye ọrụ maka heliX+ 1X BUFFER C PH 8.0 Coupling Buffer (nọmba iwu: BU-C-150-1 v2.1) nke Dynamic Biosensors GmbH & Inc. Mụta maka ihe mejupụtara ya, ntuziaka nchekwa, na ntuziaka ojiji ngwaahịa maka nanolever dị mma. njikọ.
Chọpụta 10X Passivation Solution site Dynamic Biosensors, ihe nlereanya SOL-PAS-1-5 v2.1, nke e mere maka mgbawa elu mgbawa na ngwongwo thiol-reactive. Mụta ka ị ga-esi na-atụgharị nke ọma na mezie ihe ngwọta maka nsonaazụ kacha mma.
Chọpụta ntuziaka zuru ezu na nkọwapụta maka AS-1 Adapter Strand 1 (AS-1-Rc) nke Dynamic Biosensors GmbH & Inc. Mụta maka itinye uche ya, njirimara ndị bụ isi, ntuziaka nchekwa, na ojiji maka ịrụ ọrụ biochip. Nyochaa oke agwakọta, usoro nnabata, na ihe ndị ọzọ achọrọ maka ịrụ ọrụ kacha mma. Chọpụta otu esi akwado ma chekwaa ngwaahịa a nke ọma maka ngwa ngwa ngwa ngwa ngwa.
Kwalite njupụta ligand site na DC-0 v5.1 Kit Control Density from Dynamic Biosensors. Nweta ezigbo ọdịiche ligand n'elu ihe mmetụta site na iji eriri cAnchor. Kachasị mma maka heliX+ Adapter Chip Spot 1 na Spot 2. Ntuziaka nchekwa na ntuziaka ojiji gụnyere.
The heliX + AMINE COUPLING KIT 3 nke Dynamic Biosensors GmbH na-enyere aka ijikọ ụmụ irighiri ihe pI dị ala na amines bụ isi maka ịdị ọcha proFIRE. Akwụkwọ ntuziaka onye ọrụ a na-enye ntuziaka zuru ezu maka mgbanwe DNA, njikọ njikọ ligand, na usoro nchacha. N'ikwekọ na ụdị HK-NHS-3 v6.1, ngwa a na-enye usoro nhazi usoro 3 nwere nnukwu ịdị ọcha.
